Urodynamic Events: Visualization by Dye

To visualize the urine in the pelvis, we have used a technique introduced by Steinhausen in 1964. When the dye Lissamine Green SF is injected intravenously, it quickly reaches the capillaries of the kidney and is filtered by the glomeruli. It soon appears in the proximal tubules on the cortex of the kidney. As it moves through the proximal tubule convolutions, the color fades from the first convolutions as others become green. As the dye enters the loops of Henle, the color slowly disappears from the proximal convolutions. The colored fluid then returns to the distal tubules. Since there has been water reabsorption from the renal tubules, the dye is more concentrated and appears darker.

Bolus Injection of Dye: the  whole kidney
(32.7 MB)

In the kidney papilla, filmed through the transparent pelvic wall, a similar sequence is seen. Due to the peristaltic contraction of the muscular pelvic wall, the papilla inside the pelvis is being milked by the pelvis. The dye first appears in the capillaries and the vasa recta.

Bolus Injection of Dye: the  papilla
(10.9 MB)

After it has been filtered and moved through the proximal convolutions, it appears in the loops of Henle. Not all loops fill with the dye at the same time. Gradually the dye is cleared from the loops, and after it has moved through the distal tubules, it enters the collecting tubules and the collecting ducts.

Bolus Injection of Dye: the  loops
(11.4 MB)

If dye is infused continuously, the urine moving through the collecting ducts will remain green. We can now study the movement of the urine in the collecting ducts and in the pelvic space surrounding the renal papilla.

Continuous Infusion
(9.4  MB)